ICB Cluj has initiated a key experiment within the PredAqua project by collecting approximately 7,000 L of water from Lake 3 (Gheorgheni, Cluj-Napoca), used to fill 7 mesocosms. A total of 32 types of perturbations are being tested on natural aquatic communities through the controlled addition of phytoplankton, zooplankton, and bacteria. The results will contribute to the development of predictive models aimed at understanding how aquatic ecosystems respond to environmental change.